DF-1 Cell Low Serum Medium AEM

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  • The DF-1 cell line is spontaneously immortalized, can multiply indefinitely, and has a cellular morphology typical of fibroblasts.The DF-1 cells are reverse transcriptase-negative, free of endogenous gene sequences from avian sarcoma and leukemia viruses, and are non-tumorigenic.The DF-1 cell line is a globally commercialized cell line licensed by the U.S. Food and Drug Administration (FDA).

  • It is now widely used in the proliferation of avian viruses, expression of recombinant proteins, research on oncolytic viruses, and production of animal and human vaccines.DF-1 cells are susceptible to chicken infectious bursal virus, echinoid virus, avian leukemia virus, Laurasia sarcoma virus, and turkey herpesvirus, and can replicate on DF-1 cells, and they can form obvious cytopathic lesions.

  • This cell line is an adherent-dependent cell, which is normally cultured using basal medium supplemented with fetal bovine serum, with D/F1:1 + 10% fetal bovine serum being optimal. After optimization screening, an AEM medium suitable for DF-1 cell culture was identified. The medium was chemically defined and only required the addition of 3% neonatal bovine serum to maintain DF-1 cell growth. The medium supports serum-free inoculation of viruses and can be used as a serum-free virus maintenance solution to support the proliferation of certain viruses.

  • Product Number

    Product Specification

    D21801-2

    10L/bag

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