LMH Pure Suspension Cell Culture Medium LMH-SUS III

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  • Fowl adenovirus (FAdV) can cause typical hepatitis-pericardial effusion syndrome in chickens, characterized by loss of appetite, head and neck tilt, and death within 1-2 days. It has spread rapidly since the outbreak in China in 2015, and now it has become a nationwide disease, bringing huge economic losses to the poultry industry in China.

    Chicken hepatoma (LMH) cells, the host cells for avian adenovirus (FAdV), have also been approved as a production cell line for avian adenovirus vaccines. At present, we have domesticated LMH cell suspension and developed corresponding serum-free suspension medium, and have enlarged the 1000L bioreactor to achieve a cell density of 12x106cells/ml, a passaging ratio of not less than 1:4, and a viral titer of 10.5 log TCID50/mL. Compared with the production process of adherence culture in rotary flasks, the amplification of the cell culture is simple, with high viral potency and batch production. Compared with the transflask wall culture production process, the cell culture amplification process is simple, with high viral potency, large batch production scale, good stability, and energy and operator saving. Especially after the decrease of serum usage, the production cost is reduced, and the side effects in animals after immunization are reduced.

  • Fowl adenovirus (FAdV) can cause typical hepatitis-pericardial effusion syndrome in chickens, characterized by loss of appetite, head and neck tilt, and death within 1-2 days. It has spread rapidly since the outbreak in China in 2015, and now it has become a nationwide disease, bringing huge economic losses to the poultry industry in China.

    Chicken hepatoma (LMH) cells, the host cells for avian adenovirus (FAdV), have also been approved as a production cell line for avian adenovirus vaccines. At present, we have domesticated LMH cell suspension and developed corresponding serum-free suspension medium, and have enlarged the 1000L bioreactor to achieve a cell density of 12x106cells/ml, a passaging ratio of not less than 1:4, and a viral titer of 10.5 log TCID50/mL. Compared with the production process of adherence culture in rotary flasks, the amplification of the cell culture is simple, with high viral potency and batch production. Compared with the transflask wall culture production process, the cell culture amplification process is simple, with high viral potency, large batch production scale, good stability, and energy and operator saving. Especially after the decrease of serum usage, the production cost is reduced, and the side effects in animals after immunization are reduced.

  • Fowl adenovirus (FAdV) can cause typical hepatitis-pericardial effusion syndrome in chickens, characterized by loss of appetite, head and neck tilt, and death within 1-2 days. It has spread rapidly since the outbreak in China in 2015, and now it has become a nationwide disease, bringing huge economic losses to the poultry industry in China.

    Chicken hepatoma (LMH) cells, the host cells for avian adenovirus (FAdV), have also been approved as a production cell line for avian adenovirus vaccines. At present, we have domesticated LMH cell suspension and developed corresponding serum-free suspension medium, and have enlarged the 1000L bioreactor to achieve a cell density of 12x106cells/ml, a passaging ratio of not less than 1:4, and a viral titer of 10.5 log TCID50/mL. Compared with the production process of adherence culture in rotary flasks, the amplification of the cell culture is simple, with high viral potency and batch production. Compared with the transflask wall culture production process, the cell culture amplification process is simple, with high viral potency, large batch production scale, good stability, and energy and operator saving. Especially after the decrease of serum usage, the production cost is reduced, and the side effects in animals after immunization are reduced.

  • Product Number

    Product Specification

    LM24604-2

    10L/bag

    LM24604-4

    50L/bag

    LM14604-1000

    1L/bottle


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